Reconciling Estimates of Cell Proliferation from Stable Isotope Labeling Experiments
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Reconciling Estimates of Cell Proliferation from Stable Isotope Labeling Experiments
Stable isotope labeling is the state of the art technique for in vivo quantification of lymphocyte kinetics in humans. It has been central to a number of seminal studies, particularly in the context of HIV-1 and leukemia. However, there is a significant discrepancy between lymphocyte proliferation rates estimated in different studies. Notably, deuterated (2)H2-glucose (D2-glucose) labeling stud...
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Background: Possibility to trace-label albumin with isotopes results in information concerning its synthesis, breakdown, and distribution in the intra and extra cellular spaces. The iodination of albumin is a widespread procedure used in scientific studies. Bromine not only is more reactive and less expensive than iodine, but bonds more easily with many elements. Therefore, it could be a suitab...
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Proteomics, the analysis of the proteins expressed by a cell, tissue or organism under a specific set of conditions, has undergone a tremendous period of growth in the past few years. Proteomic studies are typically designed to analyze hundreds or thousands of proteins in a single analysis and aim to provide a global view of changes in protein expression that occur in different cellular growth ...
متن کاملStable-isotope labeling by amino acids in cell culture (SILAC)
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متن کاملStable Isotope Labeling of Mammals (SILAM).
INTRODUCTIONA general approach in quantitative mass spectrometry is to mix a protein sample containing only natural-abundance isotopes with an identical protein sample containing proteins labeled with heavy stable isotopes (e.g., (2)H, (13)C, (15)N, or (18)O). Introduction of stable isotope labels into proteins alters their molecular weight and such changes can be observed on the mass spectrome...
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ژورنال
عنوان ژورنال: PLOS Computational Biology
سال: 2015
ISSN: 1553-7358
DOI: 10.1371/journal.pcbi.1004355